The goal is to identify, describe, and optimise the growth conditions for Actinobacteria mass multiplication.
The research was conducted in the Department of Agricultural Microbiology, GKVK, University of Agricultural Sciences, Bangalore, during the academic year 2019-20.
Methodology: Actinobacterial isolates were morphologically described and tested for growth factors such as pH, temperature, salt content, and carbon source usage for mass multiplication.
Results: Forty actinobacterial isolates were found in the rhizosphere soil of finger millet and cowpea, as well as in various organic manures. Most actinobacterial isolates had white, grey, or cream aerial mycelium with a dry, cottony, or powdery appearance. Gram positive, non-acid producing, and motile, all forty isolates were found. The findings of the adjustment of growth conditions revealed that all of the actinobacterial isolates grew well at 30°C, pH 7, and 2% NaCl content. During carbon source utilisation ability, starch was proven as the optimum carbon source for all actinobacterial isolates.
Conclusion: All of the actinobacterial isolates enumerated were aerobic, spore-producing, Gram positive bacteria, non-acid forming, and motile, according to the findings. During the optimization of nutritional and cultural characterisation investigations, maximum growth of Actinobacterial isolates was obtained at 30°C, pH 7, and 2% NaCl concentration with the capacity to grow on ten different carbon sources. During the evaluation of carbon source consumption abilities, starch was established as the optimum carbon source for all isolates.
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