Aims: Natural products have gotten a lot of attention in recent years for cancer prevention because of their varied health advantages, absence of toxicity, and side effects. While mounting data suggests that 18-glycyrrhetinic acid (GRA) has antiproliferative and apoptotic effects on a variety of cancer cell lines, its involvement in cervical cancer is uncertain. As a result, the current study was carried out to demonstrate GRA's cytotoxic activity against the HPV18 + human cervical carcinoma HeLa cell line. MTT and Trypan blue dye exclusion assays were used to investigate the effect of GRA on the HeLa cell line. After PI labelling, flow cytometry was used to analyse the cell cycle. Flow cytometry was used to determine apoptosis following double labelling with annexin V and PI. Caspase activation was investigated using the Caspase Activation Assay Kit. After DCFDA dye staining, reactive oxygen species (ROS) production was quantified using a fluorimeter. GRA exposure dramatically reduced the viability of HeLa cells in a dose- and time-dependent manner, according to the findings of this study. GRA stopped HeLa cells from growing in the G0/G1 phase of the cell cycle. Furthermore, as evidenced by caspase-3 and -9 activation, GRA's antiproliferative effect was mediated by apoptosis. GRA-induced caspase activation was prevented by caspase inhibitors, which reduced GRA-induced cytotoxicity. This suggested a function for the GRA-stimulated intrinsic apoptotic pathway. GRA caused a dose-related increase in ROS production in the intracellular ROS generation experiment. GRA-induced cell cycle arrest and death were totally abolished when HeLa cells were co-cultured with N-acetyl cysteine (NAC), a ROS inhibitor. As a result, the impact of NAC revealed that intracellular ROS were involved in the GRA-induced cytotoxicity.
Conclusion: GRA displayed substantial antiproliferative and apoptotic capabilities, suggesting that it might be used to help prevent and treat cervical cancer.
Please click here:https://journaljpri.com/index.php/JPRI/article/view/30823
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