The use of plant extracts in the biogenic production of silver nanoparticles (AgNPs) has proven to be a potential alternative to the traditional chemical synthesis approach. The goal of this research is to establish a simple, cost-effective, and environmentally friendly technique for production of silver nanoparticles (AgNPs) at room temperature employing seed aqueous extract of (Pimpinella anisum L.) as a reducing agent. UV visible spectroscopy, high-resolution transmission electron microscopy (HR-TEM), Fourier transform infrared (FTIR) spectroscopy, and selected area electron diffraction were used to characterise AgNPs (SAED). The phytochemicals responsible for the reduction and capping of biosynthesized AgNPs were identified by phytochemical research. The antibacterial activity of AgNPs was tested against a number of important phytopathogenic bacteria (Agrobacterium tumefaciens, Erwinia amylovora, Pectobacterium carotovorum subsp. carotovorum, Pseudomonas lachrymans, Ralstonia solanacearum) as well as Pseudomonas tolaasii, a Gram-negative bacteria that (Agaricus bisporus). The synthesised silver colloidal solution's UV-Vis spectrum revealed a strong absorption band at 441 nm, and HR-TEM analysis revealed that AgNPs were spherical with an average size of 15 - 37 nm. Furthermore, the synthesised silver nanoparticles showed strong antibacterial activity against all tested pathogenic bacteria, as measured by the inhibition zone using the agar well diffusion method. The results showed that employing plant extract to synthesise AgNPs is a potential strategy for agricultural applications.
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