Background: Research on the virulence factors of C. glabrata sensu stricto strains is becoming more common. This is attributed in part to an increase in recurring C. glabrata infections, which has resulted in higher death rates. There are very little published data on the virulence features of C. glabrata strains in Cameroon.
The purpose of this study was to evaluate various virulence features, such as the ability to build biofilms and hydrolytic enzymes (protease, esterase and phospholipase).
Methods: The current investigation included 54 non-duplicate C. glabrata sensu stricto clinical isolates that had been identified molecularly (MALDI-TOF) in a prior study. Stool (S), urine (U), oro-pharyngeal (OPS), and cervico-vaginal (CVS) swabs were taken from pregnant women, diabetic patients (both kinds 1 and 2 diabetes mellitus), HIV/AIDS patients, and other patients who did not have any of these disorders. The activities of phospholipase, protease, esterase, and biofilm were measured using techniques previously reported.Our findings demonstrated that our isolates were more capable of producing phospholipase (37.04 percent) than protease (1.85 percent) or esterase (1.85 percent) (0 percent ). Phospholipase (Pz 0.7) was found in significant concentrations in oro-pharyngeal swabs (41.17 percent) from diabetes patients and pregnant women. In addition, all of our isolates formed biofilm, with the majority (74.42%) having lower (100%) biofilm forming activity than our reference strain. We urge that further research be conducted on a greater number of strains to be able to draw a meaningful conclusion about the virulence features of C. glabrata strains in the west area.
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