Introduction: Inulins are a type of natural active polysaccharide that may be found in ginseng and dangshen and are commonly employed in pharmaceuticals and functional foods. Previous investigations have not found the purifying condition of inulin from dangshen cultivated in Vietnam. As a result, the study focused on the content, purity, molecular weight, and functional group properties of inulin isolated from natural dangshen roots (Codonopsis javanica) under various purifying circumstances.
Methods: A variety of parameters, such as the type and concentration of the solvent, the temperature and timing of precipitation, and the effect of active coal, all have an impact on inulin purifying conditions. The content of inulin, fructan, and crude polysaccharide, as well as the purification degree of inulin and fructan, were all examined. Purification inulin isolated from dangshen roots (Codonopsis javanica) growing in upland Lam Vien, Vietnam, was also studied for molecular weight and functional group features.
Results: Inulin purified with ethanol had a greater purification degree than inulin purified with active coal, with 97.85a 0.84 percent and 94.05 percent, respectively. The significant ratio in crude polysaccharide was due to inulin content, as shown by FTIR and analytical findings. When compared to another solvent, inulin and fructan were the most precipitated in ethanol, for example, 80 percent and 90 percent ethanol, respectively. The content and purification degree of inulin and fructan were affected by the types and concentrations of solvent, temperature and precipitation periods, and active coal (p 0.05). The temperature has an effect on the precipitation of inulin and fructan in ethanol solvent. Inulin and fructan have molecular weights of 3,193 Da and 1,112,892 Da, respectively.
Conclusion: Inulin from natural dangshen roots found in Vietnam's Lam Vien highlands was shown to be effective in functional foods and pharmaceutics.
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