Soursop, or Annona muricata L., is an underused fruit crop species in Sri Lanka that has recently gained popularity due to its great nutritional and therapeutic value. Soursop germplasm collections are accessible across the country, and analysing genetic diversity is necessary for conservation, identifying potential lines, and breeding initiatives to go forward. The genetic diversity of 50 soursop individuals was assessed using Inter-Simple Sequence Repeat (ISSR) markers in this study. From 2017 to 2019, the research was carried out at the Department of Agriculture's Plant Genetic Resources Centre in Gannoruwa. The DNA from 50 soursop samples was extracted using the CTAB technique, and 13 Inter Simple Sequence Repeat (ISSR) Markers were used in the Polymerase Chain Reaction (PCR). The PCR results were observed using 1.5 percent Agarose gel electrophoresis and analysed with POPGENE 1.31 utilising the Biorad Gel documentation system. PCR amplification yielded 139 bands from 13 ISSR markers, 118 of which were polymorphic. While the average number of bands detected (Na) was 1.8489 and the effective allele number (Ne) was 1.5377, the polymorphic band percentage was 85 percent. The gene diversity index (h) of the Nei was 0.3079. It was discovered that the Shannon Information Index (I) was 0.4556. At an 80 percent similarity level, a dendrogram built using the UPGMA algorithm grouped the examined accessions into four primary clusters. The findings demonstrated that the examined soursop germplasms in Sri Lanka have a high level of genetic diversity. Existing genetic variety within soursop individuals will act as a germplasm bank, allowing researchers to discover and use prospective germplasm resources for conservation and future breeding initiatives in Sri Lanka.
Please click here: https://journalajahr.com/index.php/AJAHR/article/view/30084
No comments:
Post a Comment